Cellular Viability of Cryopreserved Porcine Valve According to Warm Ischemic Time / 대한흉부외과학회지

BACKGROUND: Valve replacement using cryopreserved valved homograft is increasing because of resistance of infection and excellent hemodynamics. The viability of fibroblast which is related with warm ischemic time affects the durability of implanted cryopreserved valved homograft. We evaluated how long the warm schemic time is acceptable by examining the viability of cells depending upon warm ischemic time. MATERIAL AND METHOD: 1. Retrieval of tissues; Thirty-two slaughted porcine heart and lung enblocs were stored at refrigerator(4~8 degreesC) for various time period(Warm Ischemic Time), and the heart was dissected and stored in Hartman solution at 4 degreesCfor 24 hours(Cold Ischemic Time) as the simulation of retrieval and dissection of human heart. The hearts were assigned to groups A(2 hours), B(12 hours), C(24 hours), D(36 hours) depending on warm ischemic time. 2. Sterilization; The valved homografts were sterilized in the RPMI 1640 solution with antibiotics. 3. Freezing and Storage; The homografts were freezed by computerized freezer, stored 7 days at liquid nitrogen tank, and thawed. 4. Evaluation of the viability; The viability was evaluated by Triphan blue test after warm ischemic time, after cold ischemic time and after thawing. 5. Analysis; The viability of fibroblast was analysed by pearson correlation test of SAS program. RESULT: 1. The viability between after cold ischemic time and after thawing was not different(p=0.619) for the adequacy of sterilization, freezing and thawing. 2. The viability which was evaluated after warm ischemic time, cold ischemic time and thawing, and the various warm ischemic times are strongly correlated as R is -0.857, -0.673 and -0.549 respectively. The viability of tricuspid valve is well related with the viability of aortic valve. CONCLUSION: 1. The longer the warm ischemic time, the lesser the viability of fibroblast. The viability of fibroblast after cryopreservation was decreased less 60% if the warm ischemic time was over 12 hours. 2. The method of cryopreservation is acceptable for maintaining the viability of fibroblast, and the viability of tricuspid valve may be the indicator of the viability of aortic valve. 3. However, the study for the optimal viability which is necessary to the durabiltiy of implanted valved homograft is needed.

A Clinical Observation on Disease of Pediatric Urology / 대한비뇨기과학회지

A clinical observation was made on the diseases of pediatric urology in the Department of Urology, Seoul National University Hospital during the period from January, 1965 to August, 1972. The results are as follows. 1) Of 2,174 cases hospitalized, 164 cases were pediatric urologic patient giving a rate of 7.1%. 2) The most common disease was geuitourinary anomaly giving a ratio of 51.9%. The genitourinary tumor was 11.7%, hydrocele 9.1%, G-U injury 8.4%. urinary lithiasis 5.8% and tuberculosis 3. 9%. 3) The most common involved organ was in urethra giving a ratio of 40.2%. Testicular disease was 28. 3%, renal disease 14.4%, penile disease 5. 6%. bladder disease 4.4%. 4) The most common symptoms and signs was abnormal position of urethral meatus in 38 cases. Bladder irritating symptoms were in 26 cases, scrotal swelling and absence of scrotal content in 25 cases respectively and hematuria 22 cases. 5) Of 154 cases, 120 operations were performed in 117 cases. The most common operation was chordectomy in 30 cases. Orchiopexy was performed in 20 cases, nephrectomy in 14 cases, and second stage of Denis browne operation in 8 cases.

A Clinical Observation on Injuries of Genito, Urinary Tract / 대한비뇨기과학회지

A clinical observation was made on the Injuries of Genito-Urinary Tract of the in-patient in the Department of Urology, Seoul National University Hospital during the period from January, 1968 to September, 1969. The results are as follows. 1) Of 585 cases hospitalized, 69 cases were injury of genitourinary tract, giving a rate of 11.8%. Automobile accident was the most frequent cause of the injury (30.1%) and the next was kick or blow, fall from a height etc. 2) The urethra was involved in injuries in 39.1%. the kidney in 27.6%. the bladder in 14.5% the external genitalia in 10.1% and the ureter in 8.7%. Ureteral injury was all iatrogenic 3) The age ranged from 8 years to 68 years, the most prevalent in the third decade giving a rate of 32.0% and the next in the fourth decade giving a rate of 24.7%. The sex ratio, male to female, was 5:1. 4) Operation was performed only in 3 of I9 renal injuries and in 9 of 10 bladder injuries. Eleven of 27 urethral injuries were accompanied with pelvic bone fracture. No death was observed in this study.